Application of xanthoceras sorbifolia bunge shell saponin in resisting trichophyton rubrum and extraction and purification method of xanthoceras sorbifolia bunge shell saponin
The invention discloses application of xanthoceras sorbifolia bunge shell saponin in resisting trichophyton rubrum and an extraction and purification method of the xanthoceras sorbifolia bunge shell saponin. During extraction, xanthoceras sorbifolia bunge shells are smashed, an ethanol solution with...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses application of xanthoceras sorbifolia bunge shell saponin in resisting trichophyton rubrum and an extraction and purification method of the xanthoceras sorbifolia bunge shell saponin. During extraction, xanthoceras sorbifolia bunge shells are smashed, an ethanol solution with the volume concentration of 60% is added according to the material-liquid ratio of 1: 11, ultrasonic extraction is conducted for 1.0 h at the temperature of 60 DEG C at the power of 110 w, and after filtration and concentration, the xanthoceras sorbifolia bunge shell saponin is obtained; adding water into the obtained extract, completely dissolving, extracting by using petroleum ether, ethyl acetate and n-butyl alcohol in sequence according to a ratio of 1: 1, collecting n-butyl alcohol extract, concentrating to obtain extract, dissolving and loading a sample by using methanol, enriching and purifying by using AB-8, D101 and HP-20 macroporous resins and MCI resin, eluting by using water, 30% ethanol, 50% ethanol, |
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