Tissue culture and propagation method for Euphorbia fimbriata
The invention relates to a method for tissue culture and propagation of Euphorbia fimbriata, which comprises the following steps: selecting robust, disease-free and plump-bud annual twigs as explants, using a small brush to dip in neutral washing powder to wash stems clean, and then using tap water...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | CUI JIN MENG WEIYING GUO JIAOJIAO SHEN YANPING YAN GENGXU GAO YUNRU NIU YUNPU CUI ZEHUA SU YANG |
| description | The invention relates to a method for tissue culture and propagation of Euphorbia fimbriata, which comprises the following steps: selecting robust, disease-free and plump-bud annual twigs as explants, using a small brush to dip in neutral washing powder to wash stems clean, and then using tap water to flush for 2-3 hours; the stems are treated with 75% alcohol on an ultra-clean workbench for 30 s and washed with sterile water 3-5 times, and then the stems are disinfected with 0.1% HgCl2 or 6% sodium hypochlorite; inoculating the stem segments to a primary culture axillary bud induction culture medium, and culturing for 20 days; and carrying out subculture and rooting culture in sequence. According to the method, a two-time disinfection method is adopted in the explant disinfection stage, the pollution rate is reduced, the survival rate is increased, reasonable primary, subculture and rooting culture medium formulas and culture conditions are used, and the requirements of industrial breeding can be met.
本发明涉及一 |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN115299344A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN115299344A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN115299344A3</originalsourceid><addsrcrecordid>eNrjZLANySwuLk1VSC7NKSktSlVIzEtRKCjKL0hMTyzJzM9TyE0tychPUUjLL1JwLS3IyC9KykxUSMvMTSrKTCxJ5GFgTUvMKU7lhdLcDIpuriHOHrqpBfnxqcUFicmpeakl8c5-hoamRpaWxiYmjsbEqAEARG0wfg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Tissue culture and propagation method for Euphorbia fimbriata</title><source>esp@cenet</source><creator>CUI JIN ; MENG WEIYING ; GUO JIAOJIAO ; SHEN YANPING ; YAN GENGXU ; GAO YUNRU ; NIU YUNPU ; CUI ZEHUA ; SU YANG</creator><creatorcontrib>CUI JIN ; MENG WEIYING ; GUO JIAOJIAO ; SHEN YANPING ; YAN GENGXU ; GAO YUNRU ; NIU YUNPU ; CUI ZEHUA ; SU YANG</creatorcontrib><description>The invention relates to a method for tissue culture and propagation of Euphorbia fimbriata, which comprises the following steps: selecting robust, disease-free and plump-bud annual twigs as explants, using a small brush to dip in neutral washing powder to wash stems clean, and then using tap water to flush for 2-3 hours; the stems are treated with 75% alcohol on an ultra-clean workbench for 30 s and washed with sterile water 3-5 times, and then the stems are disinfected with 0.1% HgCl2 or 6% sodium hypochlorite; inoculating the stem segments to a primary culture axillary bud induction culture medium, and culturing for 20 days; and carrying out subculture and rooting culture in sequence. According to the method, a two-time disinfection method is adopted in the explant disinfection stage, the pollution rate is reduced, the survival rate is increased, reasonable primary, subculture and rooting culture medium formulas and culture conditions are used, and the requirements of industrial breeding can be met.
本发明涉及一</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; NEW PLANTS OR PROCESSES FOR OBTAINING THEM ; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ; TRAPPING</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20221108&DB=EPODOC&CC=CN&NR=115299344A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20221108&DB=EPODOC&CC=CN&NR=115299344A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>CUI JIN</creatorcontrib><creatorcontrib>MENG WEIYING</creatorcontrib><creatorcontrib>GUO JIAOJIAO</creatorcontrib><creatorcontrib>SHEN YANPING</creatorcontrib><creatorcontrib>YAN GENGXU</creatorcontrib><creatorcontrib>GAO YUNRU</creatorcontrib><creatorcontrib>NIU YUNPU</creatorcontrib><creatorcontrib>CUI ZEHUA</creatorcontrib><creatorcontrib>SU YANG</creatorcontrib><title>Tissue culture and propagation method for Euphorbia fimbriata</title><description>The invention relates to a method for tissue culture and propagation of Euphorbia fimbriata, which comprises the following steps: selecting robust, disease-free and plump-bud annual twigs as explants, using a small brush to dip in neutral washing powder to wash stems clean, and then using tap water to flush for 2-3 hours; the stems are treated with 75% alcohol on an ultra-clean workbench for 30 s and washed with sterile water 3-5 times, and then the stems are disinfected with 0.1% HgCl2 or 6% sodium hypochlorite; inoculating the stem segments to a primary culture axillary bud induction culture medium, and culturing for 20 days; and carrying out subculture and rooting culture in sequence. According to the method, a two-time disinfection method is adopted in the explant disinfection stage, the pollution rate is reduced, the survival rate is increased, reasonable primary, subculture and rooting culture medium formulas and culture conditions are used, and the requirements of industrial breeding can be met.
本发明涉及一</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</subject><subject>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</subject><subject>TRAPPING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLANySwuLk1VSC7NKSktSlVIzEtRKCjKL0hMTyzJzM9TyE0tychPUUjLL1JwLS3IyC9KykxUSMvMTSrKTCxJ5GFgTUvMKU7lhdLcDIpuriHOHrqpBfnxqcUFicmpeakl8c5-hoamRpaWxiYmjsbEqAEARG0wfg</recordid><startdate>20221108</startdate><enddate>20221108</enddate><creator>CUI JIN</creator><creator>MENG WEIYING</creator><creator>GUO JIAOJIAO</creator><creator>SHEN YANPING</creator><creator>YAN GENGXU</creator><creator>GAO YUNRU</creator><creator>NIU YUNPU</creator><creator>CUI ZEHUA</creator><creator>SU YANG</creator><scope>EVB</scope></search><sort><creationdate>20221108</creationdate><title>Tissue culture and propagation method for Euphorbia fimbriata</title><author>CUI JIN ; MENG WEIYING ; GUO JIAOJIAO ; SHEN YANPING ; YAN GENGXU ; GAO YUNRU ; NIU YUNPU ; CUI ZEHUA ; SU YANG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN115299344A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2022</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</topic><topic>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</topic><topic>TRAPPING</topic><toplevel>online_resources</toplevel><creatorcontrib>CUI JIN</creatorcontrib><creatorcontrib>MENG WEIYING</creatorcontrib><creatorcontrib>GUO JIAOJIAO</creatorcontrib><creatorcontrib>SHEN YANPING</creatorcontrib><creatorcontrib>YAN GENGXU</creatorcontrib><creatorcontrib>GAO YUNRU</creatorcontrib><creatorcontrib>NIU YUNPU</creatorcontrib><creatorcontrib>CUI ZEHUA</creatorcontrib><creatorcontrib>SU YANG</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>CUI JIN</au><au>MENG WEIYING</au><au>GUO JIAOJIAO</au><au>SHEN YANPING</au><au>YAN GENGXU</au><au>GAO YUNRU</au><au>NIU YUNPU</au><au>CUI ZEHUA</au><au>SU YANG</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Tissue culture and propagation method for Euphorbia fimbriata</title><date>2022-11-08</date><risdate>2022</risdate><abstract>The invention relates to a method for tissue culture and propagation of Euphorbia fimbriata, which comprises the following steps: selecting robust, disease-free and plump-bud annual twigs as explants, using a small brush to dip in neutral washing powder to wash stems clean, and then using tap water to flush for 2-3 hours; the stems are treated with 75% alcohol on an ultra-clean workbench for 30 s and washed with sterile water 3-5 times, and then the stems are disinfected with 0.1% HgCl2 or 6% sodium hypochlorite; inoculating the stem segments to a primary culture axillary bud induction culture medium, and culturing for 20 days; and carrying out subculture and rooting culture in sequence. According to the method, a two-time disinfection method is adopted in the explant disinfection stage, the pollution rate is reduced, the survival rate is increased, reasonable primary, subculture and rooting culture medium formulas and culture conditions are used, and the requirements of industrial breeding can be met.
本发明涉及一</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN115299344A |
| source | esp@cenet |
| subjects | AGRICULTURE ANIMAL HUSBANDRY FISHING FORESTRY HUMAN NECESSITIES HUNTING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES TRAPPING |
| title | Tissue culture and propagation method for Euphorbia fimbriata |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T05%3A24%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=CUI%20JIN&rft.date=2022-11-08&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN115299344A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |