Tissue culture and propagation method for Euphorbia fimbriata

The invention relates to a method for tissue culture and propagation of Euphorbia fimbriata, which comprises the following steps: selecting robust, disease-free and plump-bud annual twigs as explants, using a small brush to dip in neutral washing powder to wash stems clean, and then using tap water to flush for 2-3 hours; the stems are treated with 75% alcohol on an ultra-clean workbench for 30 s and washed with sterile water 3-5 times, and then the stems are disinfected with 0.1% HgCl2 or 6% sodium hypochlorite; inoculating the stem segments to a primary culture axillary bud induction culture medium, and culturing for 20 days; and carrying out subculture and rooting culture in sequence. According to the method, a two-time disinfection method is adopted in the explant disinfection stage, the pollution rate is reduced, the survival rate is increased, reasonable primary, subculture and rooting culture medium formulas and culture conditions are used, and the requirements of industrial breeding can be met. 本发明涉及一