Animal-free dietary collagen

Provided herein are non-naturally occurring polypeptides comprising a collagen fragment sequence and recombinant cells containing a heterologous nucleic acid sequence encoding the non-naturally occurring polypeptides. Further provided herein are animal-free methods of generating and purifying such non-naturally occurring polypeptides using microorganisms, preferably from bacterial cells. 本文提供了包含胶原蛋白片段序列的非天然存在的多肽以及含有编码该非天然存在的多肽的异源核酸序列的重组细胞。本文进一步提供了使用微生物、优选从细菌细胞生成和纯化此类非天然存在的多肽的无动物方法。