Mouse model for accurately researching iron metabolism related macrophages, construction method and application

The invention relates to a mouse model construction method for accurately researching iron metabolism related macrophages, which comprises the following steps: preparing a gene knock-in model mouse by adopting an EGE system developed by adopting a CRISPR/Cas9 technology, inserting EYFP and iCre behind a mouse Spic gene, and connecting by utilizing P2A; p2A-EYFP-P2A-iCre is inserted between a gene coding region and a 3 'UTR (Untranslated Region), and coexpression of EYFP, iCre and an indigenous gene Spic is realized through P2A connection, so that the mouse model for accurately researching the iron metabolism-related macrophages is obtained; the mouse model has complete Spic gene expression and Cre enzyme activity, can edit genes of various iron metabolism related macrophages, and is high in EYFP fluorescence intensity and high in detection result repeatability. 用于精准研究铁代谢相关巨噬细胞的小鼠模型构建方法，包括，采用CRISPR/Cas9技术开发的EGE系统制备基因敲进模式小鼠，在小鼠Spic基因后插入EYFP和iCre，并利用P2A进行连接；P2A-EYFP-P2A-iCre被插入到基因编码区和3'UTR之间，通过P2A连接实现EYFP、iCre与本