Method for improving yield of wheat anther culture green seedlings and differential culture medium
The invention relates to a method and a differential culture medium for increasing yield of wheat anther culture green seedlings, the differential culture medium comprises major elements, trace elements, ferric salt, KT, NAA, sucrose and plant gel, the concentration of the sucrose is 5-15g/L, the co...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention relates to a method and a differential culture medium for increasing yield of wheat anther culture green seedlings, the differential culture medium comprises major elements, trace elements, ferric salt, KT, NAA, sucrose and plant gel, the concentration of the sucrose is 5-15g/L, the concentration of the KT is 1.5-1.6 mg/L, and the concentration of the NAA is 0.18-0.20 mg/L. Experiments prove that wheat calluses obtained by anther culture are transferred to the differential culture medium, the yield of the wheat anther culture green seedlings is increased, and the yield of the wheat anther culture green seedlings is increased. Plants are differentiated and cultured under the conditions that the temperature is 23-25 DEG C and illumination is conducted for 14-16 hours every day, the green seedling yield is obviously increased, the green seedling differentiation rate is increased by completely removing various vitamins, glycine and other organic components in the differentiation culture medium, redu |
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