Solid tumor cell drug sensitivity detection method

The invention discloses a solid tumor cell drug sensitivity detection method. The method comprises the following steps: S1, collecting peripheral blood; s2, separating tumor platelets, circulating tumor cells and tumor stem cells in the peripheral blood; s3, collagen is added into the separated tumo...

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Hauptverfasser: ZHU RUNZHI, WANG HAIBO, PAN YAN, LIANG JUFANG, YANG HONGWEI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a solid tumor cell drug sensitivity detection method. The method comprises the following steps: S1, collecting peripheral blood; s2, separating tumor platelets, circulating tumor cells and tumor stem cells in the peripheral blood; s3, collagen is added into the separated tumor platelets, circulating tumor cells and tumor stem cells for three-dimensional culture; s4, adding a medicine to be detected, and incubating in the cell incubator; and S5, identifying the form and growth condition of the cells by adopting a drug sensitivity detection technology to obtain drug sensitivity information of the anti-tumor drug. Through the mode, the sensitivity of tumor cells to one or more drugs is detected by adopting a CCK-8 method, so that the drug sensitivity detection is standardized and commercialized, the operation of detection personnel is very convenient, and clinical individualized drug use is really realized. 本发明公开了一种实体肿瘤细胞药物敏感性检测方法,包括以下步骤:S1、采集外周血液;S2、分离外周血液中的肿瘤血小板、循环肿瘤细胞、肿瘤干细胞;S3、将分离出的肿瘤血