Primer, kit and method for detecting fowl adenovirus through fluorescent quantitative PCR (Polymerase Chain Reaction)

The invention discloses a primer, a kit and a method for detecting fowl adenovirus by fluorescent quantitative PCR (Polymerase Chain Reaction), which are characterized in that excessive fluorescent dye is added in PCR reaction, and the dye is combined with DNA double-stranded small grooves, does not emit fluorescence in a free state, and only emits light after being doped with DNA; therefore, the fowl adenovirus can be rapidly and accurately detected with the increase of the fluorescence intensity in a PCR system along with the increase of PCR products, and the dye fluorescent quantitative PCR has the advantages of high specificity, strong sensitivity and short reaction time, and can be used for relatively quantitatively comparing the virus contents of different samples. 本发明公开了一种荧光定量PCR检测禽腺病毒的引物、试剂盒及方法，本发明通过在PCR反应中加入过量荧光染料，该染料与DNA双链小沟结合，且游离状态不发荧光，只有与DNA摻入后才发光，因此在PCR体系中随着PCR产物的增加荧光强度加强可快速准确检测禽腺病毒，且染料荧光定量PCR特异性高、灵敏度强、反应时间短，可进行相对定量对比不同样本病毒含量。