Kit for detecting tumor driver gene TP53 R248W

The invention provides a kit for detecting a tumor driver gene TP53R248W, which is characterized in that a R248W mutant base is designed at the 5'tail end of a spacer sequence of crRNA by using the crRNA with a nucleotide sequence as shown in SEQ ID NO: 5, and a new mismatched base C is introduced, when two bases are mismatched between the spacer sequence of the crRNA and wild RNA, a compound cannot be formed, and Cas13a protein cannot be activated. The kit provided by the invention can be used for detecting mixed plasmids with the mutation frequency of 0.01% at least and TP53R248W variants with the concentration of 10 < 4 > copies/[mu] L in plasma at least. The kit disclosed by the invention has the advantages of good sensitivity, high specificity, simplicity and convenience in operation, rapidness and high efficiency. 本发明提供一种检测肿瘤驱动基因TP53R248W的试剂盒，用核苷酸序列如SEQ ID NO：5所示的crRNA，将R248W突变碱基设计在crRNA的间隔序列的5'末端，并引入新的错配碱基C，当crRNA的间隔序列与野生型RNA之间有两个碱基错配，将不会形成复合物，不能激活Cas13a蛋白。本发明的试剂盒最低可以检测出突变频率为0.01％的混合质粒，最低能检测出血浆中浓度为104c