Construction method and application of nasopharynx cancer PDX model

The invention discloses a construction method and application of a nasopharyngeal carcinoma PDX model, and the construction method comprises the following steps: collecting tumor tissues, and inoculating the tumor tissues to a mouse; the method comprises the following steps: constructing a P0-genera...

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Bibliographische Detailangaben
Hauptverfasser: LUO YILING, TANG LINQUAN, ZHONG QIAN, ZENG MUSHENG, CHEN MINGYUAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a construction method and application of a nasopharyngeal carcinoma PDX model, and the construction method comprises the following steps: collecting tumor tissues, and inoculating the tumor tissues to a mouse; the method comprises the following steps: constructing a P0-generation PDX model, observing the construction condition of a nasopharyngeal carcinoma PDX model, measuring the size of a tumor body, calculating the volume of the tumor, sampling and fixing tumor tissues, and then carrying out morphological index detection. And a passage model mouse can be obtained through further passage. The steps of sample pretreatment, cell digestion and the like are not involved in the method, and after the primary nasopharyngeal carcinoma PDX is successfully established, the success rate of the primary nasopharyngeal carcinoma PDX transmitted to the first-generation mouse is 58%; compared with the prior art, the method has the advantages that the planting time is shortened, the planting steps ar