Real-time fluorescent loop-mediated isothermal amplification detection system for African swine fever virus

The invention discloses an African swine fever virus real-time fluorescent loop-mediated isothermal amplification detection system, which is characterized in that after a mixed reaction reagent and a DNA polymerase solution are prepared by a reaction system construction module, the mixed reaction reagent, DNA polymerase and an amplification template are added into the same PCR tube and are uniformly mixed to construct a reaction system containing multiple mixtures; the positive control template and ultrapure water are respectively used as positive control and negative control, the detection result of the tissue sample is judged through a fluorescence value, and the detection method verification and analysis module verifies the specificity and sensitivity of the detection system to the African swine fever virus. Compared with a conventional PCR detection system, the detection system has the advantages that the LAMP primers aim at six sections of a template, the specificity of African swine fever virus detectio