Method for obtaining tumor free protein

The invention discloses a method for obtaining tumor free protein. The method comprises the following steps: S1, culturing solid tumor cells and corresponding normal tissues to obtain primary cells or performing organ culture on solid tumors and corresponding normal tissues; s2, culturing the primary cells or organs obtained in the step S1, preparing a conditioned medium, and performing polyacrylamide gel electrophoresis and nano-liter liquid chromatography electrospray tandem mass spectrometer analysis to obtain differential proteins; and S3, detecting the differential protein in the step S2 in the plasma of the solid tumor patient and the plasma of the normal person, and determining the solid tumor related free protein. Through the mode, a group of solid tumor-related free proteins can be rapidly and accurately obtained, the obtained solid tumor-related free proteins can be used for preparing antibodies, and the antibodies can be further prepared into ELISA kits or protein chips for diagnosis of solid tumor