Cell preservation method
And obtaining a cell suspension with a low cryoprotectant concentration. A cell preservation method including the following steps is used. A step (a) for concentrating the cells from a cell suspension containing the cells and a cryoprotectant to produce a concentrated component; and (b) a step for f...
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creator | YANAGIDA YASUTOMO ISHIDAO TAKEFUMI MINAMI ITSUNARI LI JIAZHONGHAO |
description | And obtaining a cell suspension with a low cryoprotectant concentration. A cell preservation method including the following steps is used. A step (a) for concentrating the cells from a cell suspension containing the cells and a cryoprotectant to produce a concentrated component; and (b) a step for freezing the concentrated component to produce a frozen product. Alternatively, a cell suspension production method comprising the following steps can be used. A step (a) for concentrating the cells from a cell suspension containing the cells and a cryoprotectant to produce a concentrated component; a step (b) for freezing the concentrated component to produce a frozen product; step (c): a step for thawing the frozen product to produce a thawed product; and (d) mixing the thawed product with a solution to produce a cell suspension.
取得冷冻保护剂浓度低的细胞悬液。使用包括以下工序的细胞的保存方法。工序(a):从含有细胞和冷冻保护液的细胞悬液浓缩细胞,生成浓缩组分;工序(b):将所述浓缩组分冷冻,生成冷冻物。或者可以使用包括以下工序的细胞悬液的生产方法工序。工序(a):从含有细胞和冷冻保护液的细胞悬液浓缩细胞,生成浓缩组分;工序(b):将所述浓缩组分冷冻,生成冷冻物;工序(c):将所述冷冻物解冻,生成 |
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取得冷冻保护剂浓度低的细胞悬液。使用包括以下工序的细胞的保存方法。工序(a):从含有细胞和冷冻保护液的细胞悬液浓缩细胞,生成浓缩组分;工序(b):将所述浓缩组分冷冻,生成冷冻物。或者可以使用包括以下工序的细胞悬液的生产方法工序。工序(a):从含有细胞和冷冻保护液的细胞悬液浓缩细胞,生成浓缩组分;工序(b):将所述浓缩组分冷冻,生成冷冻物;工序(c):将所述冷冻物解冻,生成</description><language>chi ; eng</language><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20221025&DB=EPODOC&CC=CN&NR=115244172A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20221025&DB=EPODOC&CC=CN&NR=115244172A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>YANAGIDA YASUTOMO</creatorcontrib><creatorcontrib>ISHIDAO TAKEFUMI</creatorcontrib><creatorcontrib>MINAMI ITSUNARI</creatorcontrib><creatorcontrib>LI JIAZHONGHAO</creatorcontrib><title>Cell preservation method</title><description>And obtaining a cell suspension with a low cryoprotectant concentration. A cell preservation method including the following steps is used. A step (a) for concentrating the cells from a cell suspension containing the cells and a cryoprotectant to produce a concentrated component; and (b) a step for freezing the concentrated component to produce a frozen product. Alternatively, a cell suspension production method comprising the following steps can be used. A step (a) for concentrating the cells from a cell suspension containing the cells and a cryoprotectant to produce a concentrated component; a step (b) for freezing the concentrated component to produce a frozen product; step (c): a step for thawing the frozen product to produce a thawed product; and (d) mixing the thawed product with a solution to produce a cell suspension.
取得冷冻保护剂浓度低的细胞悬液。使用包括以下工序的细胞的保存方法。工序(a):从含有细胞和冷冻保护液的细胞悬液浓缩细胞,生成浓缩组分;工序(b):将所述浓缩组分冷冻,生成冷冻物。或者可以使用包括以下工序的细胞悬液的生产方法工序。工序(a):从含有细胞和冷冻保护液的细胞悬液浓缩细胞,生成浓缩组分;工序(b):将所述浓缩组分冷冻,生成冷冻物;工序(c):将所述冷冻物解冻,生成</description><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZJBwTs3JUSgoSi1OLSpLLMnMz1PITS3JyE_hYWBNS8wpTuWF0twMim6uIc4euqkF-fGpxQWJyal5qSXxzn6GhqZGJiaG5kaOxsSoAQD-aiJY</recordid><startdate>20221025</startdate><enddate>20221025</enddate><creator>YANAGIDA YASUTOMO</creator><creator>ISHIDAO TAKEFUMI</creator><creator>MINAMI ITSUNARI</creator><creator>LI JIAZHONGHAO</creator><scope>EVB</scope></search><sort><creationdate>20221025</creationdate><title>Cell preservation method</title><author>YANAGIDA YASUTOMO ; ISHIDAO TAKEFUMI ; MINAMI ITSUNARI ; LI JIAZHONGHAO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN115244172A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2022</creationdate><topic>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>YANAGIDA YASUTOMO</creatorcontrib><creatorcontrib>ISHIDAO TAKEFUMI</creatorcontrib><creatorcontrib>MINAMI ITSUNARI</creatorcontrib><creatorcontrib>LI JIAZHONGHAO</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>YANAGIDA YASUTOMO</au><au>ISHIDAO TAKEFUMI</au><au>MINAMI ITSUNARI</au><au>LI JIAZHONGHAO</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Cell preservation method</title><date>2022-10-25</date><risdate>2022</risdate><abstract>And obtaining a cell suspension with a low cryoprotectant concentration. A cell preservation method including the following steps is used. A step (a) for concentrating the cells from a cell suspension containing the cells and a cryoprotectant to produce a concentrated component; and (b) a step for freezing the concentrated component to produce a frozen product. Alternatively, a cell suspension production method comprising the following steps can be used. A step (a) for concentrating the cells from a cell suspension containing the cells and a cryoprotectant to produce a concentrated component; a step (b) for freezing the concentrated component to produce a frozen product; step (c): a step for thawing the frozen product to produce a thawed product; and (d) mixing the thawed product with a solution to produce a cell suspension.
取得冷冻保护剂浓度低的细胞悬液。使用包括以下工序的细胞的保存方法。工序(a):从含有细胞和冷冻保护液的细胞悬液浓缩细胞,生成浓缩组分;工序(b):将所述浓缩组分冷冻,生成冷冻物。或者可以使用包括以下工序的细胞悬液的生产方法工序。工序(a):从含有细胞和冷冻保护液的细胞悬液浓缩细胞,生成浓缩组分;工序(b):将所述浓缩组分冷冻,生成冷冻物;工序(c):将所述冷冻物解冻,生成</abstract><oa>free_for_read</oa></addata></record> |
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subjects | APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE |
title | Cell preservation method |
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