Cell preservation method

And obtaining a cell suspension with a low cryoprotectant concentration. A cell preservation method including the following steps is used. A step (a) for concentrating the cells from a cell suspension containing the cells and a cryoprotectant to produce a concentrated component; and (b) a step for f...

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Bibliographische Detailangaben
Hauptverfasser: YANAGIDA YASUTOMO, ISHIDAO TAKEFUMI, MINAMI ITSUNARI, LI JIAZHONGHAO
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:And obtaining a cell suspension with a low cryoprotectant concentration. A cell preservation method including the following steps is used. A step (a) for concentrating the cells from a cell suspension containing the cells and a cryoprotectant to produce a concentrated component; and (b) a step for freezing the concentrated component to produce a frozen product. Alternatively, a cell suspension production method comprising the following steps can be used. A step (a) for concentrating the cells from a cell suspension containing the cells and a cryoprotectant to produce a concentrated component; a step (b) for freezing the concentrated component to produce a frozen product; step (c): a step for thawing the frozen product to produce a thawed product; and (d) mixing the thawed product with a solution to produce a cell suspension. 取得冷冻保护剂浓度低的细胞悬液。使用包括以下工序的细胞的保存方法。工序(a):从含有细胞和冷冻保护液的细胞悬液浓缩细胞,生成浓缩组分;工序(b):将所述浓缩组分冷冻,生成冷冻物。或者可以使用包括以下工序的细胞悬液的生产方法工序。工序(a):从含有细胞和冷冻保护液的细胞悬液浓缩细胞,生成浓缩组分;工序(b):将所述浓缩组分冷冻,生成冷冻物;工序(c):将所述冷冻物解冻,生成