Primer and probe for specifically detecting and identifying pediococcus acidilactici and application of primer and probe

The invention belongs to the technical field of biological strain detection, and relates to a primer and a probe for specifically detecting and identifying pediococcus acidilactici and application of the primer and the probe. According to the invention, two primer probes are designed and obtained on...

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Hauptverfasser: SUN LINHUI, HAN IN-HO, PENG QIAN, LI DIAN, HOU SHAOYANG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of biological strain detection, and relates to a primer and a probe for specifically detecting and identifying pediococcus acidilactici and application of the primer and the probe. According to the invention, two primer probes are designed and obtained on the basis of one strain of pediococcus acidilactici HP-B1099 through an ERIC-PCR technology, so that the trace amount of pediococcus acidilactici in a complex sample can be simply and quickly detected, and the defects of a traditional method are overcome. The pediococcus acidilactici with extremely low concentration can be rapidly and specifically identified in eight genome DNAs or a mixed solution of eight strains and a plurality of related strains, and the detection efficiency is greatly improved. The method has wide application prospects in the aspects of human and animal intestinal flora ecological structure research, medicine health, livestock breeding, commercial production and application and the like. 本发明属