Hybridoma cell strain 5B3 and orientia tsutsugamushi 56kDa protein monoclonal antibody as well as preparation method and application thereof

The invention discloses a hybridoma cell strain 5B3, an orientia tsutsugamushi 56kDa protein monoclonal antibody as well as a preparation method and application of the hybridoma cell strain 5B3 and the orientia tsutsugamushi 56kDa protein monoclonal antibody. The hybridoma cell strain 5B3 is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: C2022244. The ascites indirect ELISA titer of the 56kDa protein monoclonal antibody provided by the invention is 1: 1280000, the antibody subclass is IgG2akappa, and the 56kDa protein monoclonal antibody has the capability of stably secreting the antibody, is strong in specificity and simple in preparation method, can react with an oriental body infected strain, and can be used as a diagnostic antibody for establishing an immunological rapid detection method. 本发明公开了一种杂交瘤细胞株5B3、恙虫病东方体56kDa蛋白单克隆抗体及其制备方法与应用，所述的杂交瘤细胞株5B3保藏于中国典型培养物保藏中心，保藏编号为CCTCC NO：C2022244。本发明提供的56kDa蛋白单克隆抗体腹水间接ELISA效价为1:1280000，抗体亚类为IgG2aκ，具有稳定分泌抗体的能力