HPLC-ELSD detection method for lecithin in poultry eggs

The invention relates to an HPLC-ELSD detection method for lecithin in poultry eggs. The invention establishes a method for detecting PC in poultry eggs by high performance liquid chromatography-evaporative light scattering. The result shows that when the PC is in a range of 0.1-2.0 mg/mL, the logar...

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Bibliographische Detailangaben
Hauptverfasser:	GE QINGLIAN, CHEN DAWEI, PU JUNHUA, LIU YINYIN, FAN YANFENG, TANG XIUJUN, ZHANG JING, GAO YUSHI, TANG MENGJUN, ZHANG XIAOYAN, JIA XIAOXU, MA LINA, LU JUNXIAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PHYSICS TESTING
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Beschreibung
Zusammenfassung:	The invention relates to an HPLC-ELSD detection method for lecithin in poultry eggs. The invention establishes a method for detecting PC in poultry eggs by high performance liquid chromatography-evaporative light scattering. The result shows that when the PC is in a range of 0.1-2.0 mg/mL, the logarithm of the PC peak area and the logarithm of the corresponding mass concentration are in a good linear relationship (R2 is greater than 0.999); the relative standard deviation of precision test results is less than 2.7%, and the recovery rate is 95.0%-105.0%. 本发明涉及一种禽蛋中卵磷脂的HPLC-ELSD检测方法。本发明建立了高效液相色谱法-蒸发光散射检测禽蛋中PC的方法；结果表明，PC在0.1～2.0mg/mL范围内，PC峰面积的对数与相应质量浓度的对数呈良好的线性关系（R2＞0.999）；精密度试验结果相对标准偏差均＜2.7%，回收率为95.0%～105.0%。