Non-interference SERS probe as well as preparation method and application thereof

The invention discloses a non-interference SERS probe as well as a preparation method and application thereof, and relates to the technical field of surface enhanced Raman spectrum detection. The Raman characteristic peak of the SERS probe disclosed by the invention is within the range of 1800 cm to 2500 cm of a Raman silence zone; the SERS probe comprises a colloidal gold structure and a silver shell arranged on the surface of the colloidal gold structure, the silver shell is connected with a plurality of Raman reporter molecule phenylacetylene, the outer side of the phenylacetylene is connected with a polydopamine layer formed by auto-polymerization wrapping of dopamine, and the outer side of the polydopamine layer is connected with a procalcitonin antibody. The probe disclosed by the invention is good in stability and reproducibility and strong in optical response; the SERS probe can solve the problem that the SERS probe is easily subjected to optical interference of endogenous biomolecules in