Disinfection and sterile sowing method for ludisia discolor pods
The invention discloses a ludisia discolor pod disinfection and sterile sowing method. The method comprises the following steps: (1) collecting living plants of ludisia discolor wild germplasm from the field; (2) washing the living plants with tap water, soaking the living plants in a detergent, cle...
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Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a ludisia discolor pod disinfection and sterile sowing method. The method comprises the following steps: (1) collecting living plants of ludisia discolor wild germplasm from the field; (2) washing the living plants with tap water, soaking the living plants in a detergent, cleaning the living plants, and absorbing water to obtain clean plants; (3) cutting off fruit pods of the clean plants by using a sterile instrument, completely treating the fruit pods with alcohol and mercury bichloride, cutting the fruit pods into sections, keeping at least one bud point, and then putting the fruit pods into an induction culture medium for induction culture to obtain bottle seedlings; (4) hardening the bottle seedlings obtained in the step (1), and transplanting the hardened bottle seedlings into a transplanting substrate; and (5) spraying metalaxyl hymexazol wettable powder on the transplanted bottle seedlings. According to the method, ludisia discolor pods are adopted as the source of explants, on |
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