Batch induction method of konjak mutants

The invention discloses a batch induction method of konjak mutants, and relates to the technical field of biology. The method comprises the following steps: carrying out germination accelerating treatment on konjak rhizomes or corms, cutting the konjak rhizomes or corms into blocks, and carrying out...

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Bibliographische Detailangaben
Hauptverfasser: DUAN LONGFEI, CHEN GUO'AI, ZHANG BAIREN, QIN JIANFENG, CAI YANGGUANG
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a batch induction method of konjak mutants, and relates to the technical field of biology. The method comprises the following steps: carrying out germination accelerating treatment on konjak rhizomes or corms, cutting the konjak rhizomes or corms into blocks, and carrying out mutagenesis culture on the obtained blocks in a culture medium containing an EMS mutagenic agent; performing < 60 > Co-gamma mutagenesis treatment on the cut blocks subjected to mutagenesis culture; directly inducing adventitious buds to differentiate seedlings from the cut blocks subjected to 60Co-gamma mutagenesis treatment; carrying out rooting culture on the seedlings, then transplanting and culturing, and harvesting to obtain the small corms. According to the method disclosed by the invention, a large number of mutant individuals can be rapidly induced, the process operation is simple, the repeatability is high, the seed resource innovation abundance is high, and a rich material source is provided for directi