Taqman probe qPCR (quantitative polymerase chain reaction) detection primer group for rapidly and quantitatively detecting pathogenic vibrio alginolyticus and application

The invention discloses a Taqman probe qPCR (quantitative polymerase chain reaction) detection primer group for rapidly and quantitatively detecting pathogenic vibrio alginolyticus and application, and belongs to the field of pathogenic microorganism detection. The specific primer (toxR-F420-437 and toxR-R531-548) and the probe (toxR-P440) designed for the vibrio alginolyticus toxR are rapid, accurate and high in sensitivity, the vibrio alginolyticus can be effectively detected without enrichment, and the lowest detection limit is 2.4 * 10 < 1 > copy/microliter; pathogenic vibrio alginolyticus can be effectively distinguished from various common vibrios, and the specificity is very high; the number of pathogenic vibrio alginolyticus in a water sample and a cultured animal sample can be rapidly and directly detected, so that a scientific basis is provided for healthy culture management and disease prevention and control. 本发明公开一种快速定量检测致病性溶藻弧菌的Taqman探针qPCR检测引物组和应用，属于病原微生物检测领域。本发明设计的针对溶藻弧菌toxR特异性引物(toxR-F420-437和