Biochar liquid culture medium for culturing phosphorus-solubilizing algae and application method of biochar liquid culture medium
The invention discloses a biochar liquid culture medium for culturing phosphorus-solubilizing algae and an application method thereof, the application method comprises the following steps: (1) preparing a culture medium: weighing raw materials in proportion, adding sterile water and mixing to obtain...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a biochar liquid culture medium for culturing phosphorus-solubilizing algae and an application method thereof, the application method comprises the following steps: (1) preparing a culture medium: weighing raw materials in proportion, adding sterile water and mixing to obtain the liquid culture medium, the culture medium containing biochar; (2) sterilization: carrying out high-pressure steam sterilization treatment on the liquid culture medium obtained in the step (1), transferring the liquid culture medium to a sterile room, cooling the liquid culture medium to room temperature, and subpackaging the culture medium into culture bottles while stirring; (3) inoculation: performing inoculation operation in a sterile room; and (4) incubating: putting the inoculated culture medium into a sterile incubator, and incubating to obtain the algae liquid. According to the method, the phosphorus-solubilizing algae with high yield and stronger phosphorus-solubilizing function can be obtained by prep |
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