Preparation method of Tirzeptide

The invention relates to the field of preparation of polypeptide drugs, and discloses a preparation method of Tirzeptide, which mainly comprises the following steps: preparing Tirzeptide peptide resin by using a solid phase polypeptide synthesis method, and cracking the Tirzeptide peptide resin to obtain Tirzeptide; wherein the method for inoculating the Phe-Val-Gln-Trp-Leu is as follows: 5 to 7 peptide fragments containing the Phe-Val-Gln-Trp-Leu are adopted. According to the method disclosed by the invention, the 5-7 peptide fragments containing Phe-Val-Gln-Trp-Leu are selected for solid phase coupling, so that the generation of deleted peptides and related racemization impurities can be reduced, the purification difficulty is reduced, and the yield is improved. 本发明涉及多肽药物制备领域，公开了一种Tirzepatide的制备方法，主要包括以下步骤，用固相多肽合成法制备Tirzepatide肽树脂，Tirzepatide肽树脂经裂解得到Tirzepatide；其中接入Phe-Val-Gln-Trp-Leu的方法为：采用含Phe-Val-Gln-Trp-Leu的5～7肽片段。本发明选择含Phe-Val-Gln-Trp-Leu的5～7肽片段进行固相偶联，可以减少缺失肽以及相关消旋杂质的产生，减小纯化难度，提高收率。