Improved sandwich method immunochromatography test strip as well as preparation method and application thereof

The invention discloses an improved sandwich method immunochromatography test strip and a preparation method and application thereof, a biotin conjugate is coated on a first conjugate pad of the test strip, the biotin conjugate is formed by combining an antibody 1 or an antigen 1 with biotin, a tracing conjugate is coated on a second conjugate pad, and the tracing conjugate is formed by combining an antibody 2 or an antigen 2 with biotin. The tracing conjugate is formed by combining an antibody 2 or an antigen 2 with microsphere particles, and the detection line is coated with polymeric streptavidin. The test strip provided by the invention overcomes the defects of steric hindrance, weak binding force, short reaction time of a compound with a sandwich structure on a solid-phase carrier and insufficient adsorption amount in an original sandwich method, so that the detection sensitivity and the specificity level of an antigen to be detected are improved. 本发明公开了一种改良型的夹心法免疫层析试纸条及其制备方法和应用，该试纸条的第一层结合物垫上包被有生物素结合物，生物