Ratio-dependent up-conversion fluorescence detection device and method based on micro-fluidic chip

The invention provides a ratio type up-conversion fluorescence detection device and method based on a micro-fluidic chip, and relates to the field of fluorescence detection. The ratio-type up-conversion fluorescence detection device based on the micro-fluidic chip comprises a device body, a paper-based micro-fluidic sample introduction module, an optical path module and a detection module, two mounting grooves are formed in the device body, the paper-based micro-fluidic sample introduction module comprises a chip sample introduction sliding block, the paper-based micro-fluidic chip is mounted on the sample introduction sliding block, and the optical path module is mounted on the chip sample introduction sliding block. Near-infrared laser diodes distributed in an array mode are detachably installed on the excitation light path sliding block, and the included angle between the paper-based micro-fluidic chip and the excitation light path direction emitted by the near-infrared laser diodes is 60 degrees. The prob