Promoter element responding to submarine metal Ni < 2 + > ions, electrochemical signal output sensor prepared from promoter element and application of electrochemical signal output sensor

The invention discloses a promoter element responding to submarine metal Ni < 2 + > ions, an electrochemical signal output sensor prepared from the promoter element and application of the electrochemical signal output sensor. The nucleotide sequence of the promoter element is shown as SEQ ID N...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	MA RAN, LI MEIJIE, YANG JIANMING, WANG ZHAOBAO, TANG RUOHAO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention discloses a promoter element responding to submarine metal Ni < 2 + > ions, an electrochemical signal output sensor prepared from the promoter element and application of the electrochemical signal output sensor. The nucleotide sequence of the promoter element is shown as SEQ ID NO.2 or SEQ ID NO.3. The promoter element has the capability of remarkably improving the detection sensitivity of metal Ni < 2 + >. The promoter element is used for transcriptional expression of NAD < + > synthetase and formate dehydrogenase genes to prepare an electrochemical signal output sensor, and by increasing the total amount of intracellular electron pools (NAD < + >) and the ratio of NADH < 2 + >/NAD < + >, output of electrochemical signals after induction of submarine metal Ni < 2 + > ions is achieved. The invention provides a new thought and method for screening an efficient inducible promoter and a detection mode, and improves the accuracy, safety and efficiency of metal Ni < 2 + > detection. 本发明公开了一种响应潜艇金属Ni2