Immortalized Holstein bull calf small intestine epithelial cell line and construction method and application thereof

The invention belongs to the field of cell modification by introducing external genetic materials, and particularly relates to an immortalized Holstein bull calf small intestine epithelial cell line as well as a construction method and application of the immortalized Holstein bull calf small intesti...

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Bibliographische Detailangaben
Hauptverfasser:	HE LEI, MENG SUDAN, WANG HAIRONG, ZHANG CAI, DOU MENGYING, SHAO QI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention belongs to the field of cell modification by introducing external genetic materials, and particularly relates to an immortalized Holstein bull calf small intestine epithelial cell line as well as a construction method and application of the immortalized Holstein bull calf small intestine epithelial cell line. The immortalized Holstein bull calf small intestine epithelial cell line has a preservation number of CGMCC No.45029, and is preserved in China General Microbiological Culture Collection Center on December 27th, 2021, and the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing. The immortalized Holstein bull calf small intestine epithelial cell line has the advantages that cells obtained through screening are high in viability, high cell viability and biological characteristics can still be kept after 80 generations of passage, due to the fact that a non-virus transfection thought is adopted, the influence on the