Tissue culture and rapid propagation method of seed trichosanthes kirilowii maxim seedlings
The invention discloses a method for tissue culture and rapid propagation of seed trichosanthes kirilowii seedlings, which comprises the following steps: sterilizing and disinfecting stem tips of seed trichosanthes kirilowii as explants, culturing in a proper culture medium under proper culture cond...
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| creator | HE RONGRONG SUN YANGYANG XING SHUTANG ZHAO YITONG ZHAO FEI SUN XIAOLIN CAI HONGYUE |
| description | The invention discloses a method for tissue culture and rapid propagation of seed trichosanthes kirilowii seedlings, which comprises the following steps: sterilizing and disinfecting stem tips of seed trichosanthes kirilowii as explants, culturing in a proper culture medium under proper culture conditions, dedifferentiating and differentiating to form adventitious buds, and carrying out subculture multiplication and rooting culture to obtain test-tube plantlets. And the test-tube plantlets are transplanted, strong and hardened to obtain cultivated seedlings. By means of the method, a large number of test-tube plantlets capable of determining male and female plants can be obtained in a short period, the survival rate of the test-tube plantlets reaches 90% or above after the test-tube plantlets are transplanted, and large-area popularization of the seed trichosanthes kirilowii maxim can be rapidly achieved.
本发明公开了一种籽用栝楼种苗组培快繁的方法，该方法将籽用栝楼的茎尖作为外植体，经灭菌消毒后，在合适的培养基和培养条件下培养，使其能够脱分化并分化形成不定芽，再经继代增殖、生根培养获得试管苗，试管苗经移栽壮苗炼苗 |
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本发明公开了一种籽用栝楼种苗组培快繁的方法，该方法将籽用栝楼的茎尖作为外植体，经灭菌消毒后，在合适的培养基和培养条件下培养，使其能够脱分化并分化形成不定芽，再经继代增殖、生根培养获得试管苗，试管苗经移栽壮苗炼苗</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; NEW PLANTS OR PROCESSES FOR OBTAINING THEM ; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ; TRAPPING</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20221011&DB=EPODOC&CC=CN&NR=115152632A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20221011&DB=EPODOC&CC=CN&NR=115152632A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HE RONGRONG</creatorcontrib><creatorcontrib>SUN YANGYANG</creatorcontrib><creatorcontrib>XING SHUTANG</creatorcontrib><creatorcontrib>ZHAO YITONG</creatorcontrib><creatorcontrib>ZHAO FEI</creatorcontrib><creatorcontrib>SUN XIAOLIN</creatorcontrib><creatorcontrib>CAI HONGYUE</creatorcontrib><title>Tissue culture and rapid propagation method of seed trichosanthes kirilowii maxim seedlings</title><description>The invention discloses a method for tissue culture and rapid propagation of seed trichosanthes kirilowii seedlings, which comprises the following steps: sterilizing and disinfecting stem tips of seed trichosanthes kirilowii as explants, culturing in a proper culture medium under proper culture conditions, dedifferentiating and differentiating to form adventitious buds, and carrying out subculture multiplication and rooting culture to obtain test-tube plantlets. And the test-tube plantlets are transplanted, strong and hardened to obtain cultivated seedlings. By means of the method, a large number of test-tube plantlets capable of determining male and female plants can be obtained in a short period, the survival rate of the test-tube plantlets reaches 90% or above after the test-tube plantlets are transplanted, and large-area popularization of the seed trichosanthes kirilowii maxim can be rapidly achieved.
本发明公开了一种籽用栝楼种苗组培快繁的方法，该方法将籽用栝楼的茎尖作为外植体，经灭菌消毒后，在合适的培养基和培养条件下培养，使其能够脱分化并分化形成不定芽，再经继代增殖、生根培养获得试管苗，试管苗经移栽壮苗炼苗</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</subject><subject>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</subject><subject>TRAPPING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyrEKwjAQANAsDqL-w_kBDm2puxTFyambQzmSa3s0yYVcin6-IH6A01ve1jx7Vl0J7OrLmgkwOsiY2EHKknDCwhIhUJnFgYygRA5KZjuLYiwzKSyc2cuLGQK-OXyL5zjp3mxG9EqHnztzvF377n6iJANpQkuRytA9qqqt2vrc1Jfmn_MBxSk8IQ</recordid><startdate>20221011</startdate><enddate>20221011</enddate><creator>HE RONGRONG</creator><creator>SUN YANGYANG</creator><creator>XING SHUTANG</creator><creator>ZHAO YITONG</creator><creator>ZHAO FEI</creator><creator>SUN XIAOLIN</creator><creator>CAI HONGYUE</creator><scope>EVB</scope></search><sort><creationdate>20221011</creationdate><title>Tissue culture and rapid propagation method of seed trichosanthes kirilowii maxim seedlings</title><author>HE RONGRONG ; SUN YANGYANG ; XING SHUTANG ; ZHAO YITONG ; ZHAO FEI ; SUN XIAOLIN ; CAI HONGYUE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN115152632A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2022</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</topic><topic>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</topic><topic>TRAPPING</topic><toplevel>online_resources</toplevel><creatorcontrib>HE RONGRONG</creatorcontrib><creatorcontrib>SUN YANGYANG</creatorcontrib><creatorcontrib>XING SHUTANG</creatorcontrib><creatorcontrib>ZHAO YITONG</creatorcontrib><creatorcontrib>ZHAO FEI</creatorcontrib><creatorcontrib>SUN XIAOLIN</creatorcontrib><creatorcontrib>CAI HONGYUE</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HE RONGRONG</au><au>SUN YANGYANG</au><au>XING SHUTANG</au><au>ZHAO YITONG</au><au>ZHAO FEI</au><au>SUN XIAOLIN</au><au>CAI HONGYUE</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Tissue culture and rapid propagation method of seed trichosanthes kirilowii maxim seedlings</title><date>2022-10-11</date><risdate>2022</risdate><abstract>The invention discloses a method for tissue culture and rapid propagation of seed trichosanthes kirilowii seedlings, which comprises the following steps: sterilizing and disinfecting stem tips of seed trichosanthes kirilowii as explants, culturing in a proper culture medium under proper culture conditions, dedifferentiating and differentiating to form adventitious buds, and carrying out subculture multiplication and rooting culture to obtain test-tube plantlets. And the test-tube plantlets are transplanted, strong and hardened to obtain cultivated seedlings. By means of the method, a large number of test-tube plantlets capable of determining male and female plants can be obtained in a short period, the survival rate of the test-tube plantlets reaches 90% or above after the test-tube plantlets are transplanted, and large-area popularization of the seed trichosanthes kirilowii maxim can be rapidly achieved.
本发明公开了一种籽用栝楼种苗组培快繁的方法，该方法将籽用栝楼的茎尖作为外植体，经灭菌消毒后，在合适的培养基和培养条件下培养，使其能够脱分化并分化形成不定芽，再经继代增殖、生根培养获得试管苗，试管苗经移栽壮苗炼苗</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY FISHING FORESTRY HUMAN NECESSITIES HUNTING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES TRAPPING |
| title | Tissue culture and rapid propagation method of seed trichosanthes kirilowii maxim seedlings |
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