Tissue culture and rapid propagation method of seed trichosanthes kirilowii maxim seedlings

The invention discloses a method for tissue culture and rapid propagation of seed trichosanthes kirilowii seedlings, which comprises the following steps: sterilizing and disinfecting stem tips of seed trichosanthes kirilowii as explants, culturing in a proper culture medium under proper culture conditions, dedifferentiating and differentiating to form adventitious buds, and carrying out subculture multiplication and rooting culture to obtain test-tube plantlets. And the test-tube plantlets are transplanted, strong and hardened to obtain cultivated seedlings. By means of the method, a large number of test-tube plantlets capable of determining male and female plants can be obtained in a short period, the survival rate of the test-tube plantlets reaches 90% or above after the test-tube plantlets are transplanted, and large-area popularization of the seed trichosanthes kirilowii maxim can be rapidly achieved. 本发明公开了一种籽用栝楼种苗组培快繁的方法，该方法将籽用栝楼的茎尖作为外植体，经灭菌消毒后，在合适的培养基和培养条件下培养，使其能够脱分化并分化形成不定芽，再经继代增殖、生根培养获得试管苗，试管苗经移栽壮苗炼苗