Means and methods for leukocyte subpopulations based on multi-parameter cell counting

The present invention relates to the field of diagnostic immunology. Means and methods are provided for leukocyte subpopulations based on multi-parameter cytometry, which can be advantageously used to monitor the immune state of an individual and/or to monitor the effectiveness of an immunomodulatory therapy. Wherein a reagent composition is provided, comprising an antibody coupled to a detectable tag, the coupled antibody being directed against a combination of the following labels: CD141, HLA-DR, CD16, CD33, CD300e, CD303 and CD14, and wherein the antibodies directed against CD300e and CD303 can be coupled to the same tag. 本发明涉及诊断免疫学领域。提供了用于基于多参数细胞计数的白细胞亚群的手段和方法，可有利地用于监测一个体的免疫状态及/或监测免疫调节治疗的效果。其中提供一种试剂组合物，包括偶联到可检测标签上的抗体，所述偶联抗体针对以下标记组合：CD141、HLA-DR、CD16、CD33、CD300e、CD303和CD14，其中针对CD300e和CD303的抗体可以偶联到同一标签上。