Isolated culture method of aerial microalgae

The method is characterized by comprising the following steps: preparing a culture solution rich in an inorganic nutrient source and an organic carbon source, obtaining a liquid culture medium and a solid culture medium based on the culture solution, carrying out enrichment culture and enlarged cult...

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Bibliographische Detailangaben
Hauptverfasser: TANG BO, HU YUPENG, LEI KAIYUN, LI QI, LIU ZHEN, ZHOU LEI, YUAN FEIYUN, TANG XUEFENG, SUN OU, ZHAO HUIJUAN, ZHOU RUI, ZOU JIAN, LI CONG, XIAO FENG, GOU MINGJIA
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The method is characterized by comprising the following steps: preparing a culture solution rich in an inorganic nutrient source and an organic carbon source, obtaining a liquid culture medium and a solid culture medium based on the culture solution, carrying out enrichment culture and enlarged culture by using the liquid culture medium, and carrying out repeated separation and purification culture by using the solid culture medium. And selecting the peripheries of the algae spots with good characteristic consistency, transferring the algae spots into a liquid culture medium, carrying out enrichment culture, and carrying out purebred detection. According to the method, the aerogenic microalgae target object can be better separated from the natural environment, the growth of the aerogenic microalgae can be effectively promoted, and the influence of bacteria and fungi is reduced, so that the aerogenic microalgae is effectively separated and purified. 本发明公开了一种气生微藻的分离培养方法,其特征在于,先制备富含无机营养源和有机碳源的培养液,基于该培养液获得液体培养基和固