Method for preparing cell-free protein expression gel by using plasmid

The present invention relates to a method for preparing a cell-free protein expression gel and, more specifically, to a method for preparing a cell-free protein expression gel in which a double-stranded cyclic plasmid containing a base sequence containing genetic information for expressing a target protein is separated into a single-stranded cyclic plasmid, and the single-stranded cyclic plasmid is separated into a single-stranded cyclic plasmid. And the extended DNA chains obtained by the DNA polymerase chain reaction are complementarily bonded to each other, thereby preparing a cell-free protein expression gel. 本发明涉及用于制备无细胞的蛋白表达凝胶的方法，并且更具体而言，涉及用于制备无细胞的蛋白表达凝胶的方法，其中，将包含含有用于表达靶蛋白的遗传信息的碱基序列的双链环状质粒分离成单链环状质粒，并将通过DNA聚合酶链式反应而获得的经延长的DNA链彼此互补地结合，从而制备无细胞的蛋白表达凝胶。