Construction method and application of animal model for conditionally inducing neural stem cells to proliferate

The invention discloses a construction method and application of an animal model for conditionally inducing neural stem cells to proliferate. The construction method comprises the following steps: connecting a Yap1S96A/S352A mutant (SEQ ID NO.1) fragment to a CAGGs: LP-EGFP-LP-Chryry plasmid skeleton, so as to obtain a recombinant plasmid; the preparation method comprises the following steps of: firstly, performing micro-injection on the obtained mixture and Tol2 transposase mRNA (messenger Ribonucleic Acid) into an oosperm of the obtuse ovulus of Mexican ovulus, performing conventional feeding, then electrically transforming Cre recombinase expression plasmids, activating expression of a Yap1S96AS352A mutant by using Cre recombinase, and further inducing nerve cells to be transdifferentiated into neural stem cells and proliferating the neural stem cells. The method provided by the invention provides a powerful tool for researching the functions of the neural stem cells in vivo, especially the functions in th