Th2 cell in-vitro culture method
The invention provides a culture method for in-vitro culture of Th2 cells, and belongs to the technical field of cell culture. The method comprises the following steps: acquiring peripheral blood PBMC (peripheral blood mononuclear cell), adjusting cell density by using an ALyS505N-0 culture medium c...
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| creator | HUANG HAIJUAN YUAN MINGTAO LAN XIN KONG WEISHENG |
| description | The invention provides a culture method for in-vitro culture of Th2 cells, and belongs to the technical field of cell culture. The method comprises the following steps: acquiring peripheral blood PBMC (peripheral blood mononuclear cell), adjusting cell density by using an ALyS505N-0 culture medium containing autologous plasma, adding CD3, CD28, BCL2L2 protein, neuropeptide, morphine, doxycycline, IL-13, IL-16, IL-11, IL-4, IL-2, DAY3 and DAY5 into the DAY0, adding an ALyS505N-0 culture medium containing IL-13, IL-16, IL-11, IL-4 and IL-2, respectively adding DAY7, DAY9 and DAY11 into an ALyS505N-0 culture medium containing IL-11, IL-4 and IL-2, culturing for 14 days, and harvesting cells. According to the method, the purity and proliferation multiple of TH2 cells are remarkably improved, the purity is 70% or above, and the proliferation multiple is 300 times or above.
本发明提供了一种体外培养Th2细胞的培养方法，属于细胞培养技术领域。获取外周血PBMC，使用含自体血浆的ALyS505N-0培养基调整细胞密度，DAY0加入CD3、CD28、BCL2L2蛋白、神经肽、吗啡、多西环素、IL-13、IL-16、IL-11、IL-4、IL-2，DAY3、DA |
| format | Patent |
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本发明提供了一种体外培养Th2细胞的培养方法，属于细胞培养技术领域。获取外周血PBMC，使用含自体血浆的ALyS505N-0培养基调整细胞密度，DAY0加入CD3、CD28、BCL2L2蛋白、神经肽、吗啡、多西环素、IL-13、IL-16、IL-11、IL-4、IL-2，DAY3、DA</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220930&DB=EPODOC&CC=CN&NR=115125203A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220930&DB=EPODOC&CC=CN&NR=115125203A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HUANG HAIJUAN</creatorcontrib><creatorcontrib>YUAN MINGTAO</creatorcontrib><creatorcontrib>LAN XIN</creatorcontrib><creatorcontrib>KONG WEISHENG</creatorcontrib><title>Th2 cell in-vitro culture method</title><description>The invention provides a culture method for in-vitro culture of Th2 cells, and belongs to the technical field of cell culture. The method comprises the following steps: acquiring peripheral blood PBMC (peripheral blood mononuclear cell), adjusting cell density by using an ALyS505N-0 culture medium containing autologous plasma, adding CD3, CD28, BCL2L2 protein, neuropeptide, morphine, doxycycline, IL-13, IL-16, IL-11, IL-4, IL-2, DAY3 and DAY5 into the DAY0, adding an ALyS505N-0 culture medium containing IL-13, IL-16, IL-11, IL-4 and IL-2, respectively adding DAY7, DAY9 and DAY11 into an ALyS505N-0 culture medium containing IL-11, IL-4 and IL-2, culturing for 14 days, and harvesting cells. According to the method, the purity and proliferation multiple of TH2 cells are remarkably improved, the purity is 70% or above, and the proliferation multiple is 300 times or above.
本发明提供了一种体外培养Th2细胞的培养方法，属于细胞培养技术领域。获取外周血PBMC，使用含自体血浆的ALyS505N-0培养基调整细胞密度，DAY0加入CD3、CD28、BCL2L2蛋白、神经肽、吗啡、多西环素、IL-13、IL-16、IL-11、IL-4、IL-2，DAY3、DA</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZFAIyTBSSE7NyVHIzNMtyywpyldILs0pKS1KVchNLcnIT-FhYE1LzClO5YXS3AyKbq4hzh66qQX58anFBYnJqXmpJfHOfoaGpoZGpkYGxo7GxKgBABxdJLo</recordid><startdate>20220930</startdate><enddate>20220930</enddate><creator>HUANG HAIJUAN</creator><creator>YUAN MINGTAO</creator><creator>LAN XIN</creator><creator>KONG WEISHENG</creator><scope>EVB</scope></search><sort><creationdate>20220930</creationdate><title>Th2 cell in-vitro culture method</title><author>HUANG HAIJUAN ; YUAN MINGTAO ; LAN XIN ; KONG WEISHENG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN115125203A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2022</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>HUANG HAIJUAN</creatorcontrib><creatorcontrib>YUAN MINGTAO</creatorcontrib><creatorcontrib>LAN XIN</creatorcontrib><creatorcontrib>KONG WEISHENG</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HUANG HAIJUAN</au><au>YUAN MINGTAO</au><au>LAN XIN</au><au>KONG WEISHENG</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Th2 cell in-vitro culture method</title><date>2022-09-30</date><risdate>2022</risdate><abstract>The invention provides a culture method for in-vitro culture of Th2 cells, and belongs to the technical field of cell culture. The method comprises the following steps: acquiring peripheral blood PBMC (peripheral blood mononuclear cell), adjusting cell density by using an ALyS505N-0 culture medium containing autologous plasma, adding CD3, CD28, BCL2L2 protein, neuropeptide, morphine, doxycycline, IL-13, IL-16, IL-11, IL-4, IL-2, DAY3 and DAY5 into the DAY0, adding an ALyS505N-0 culture medium containing IL-13, IL-16, IL-11, IL-4 and IL-2, respectively adding DAY7, DAY9 and DAY11 into an ALyS505N-0 culture medium containing IL-11, IL-4 and IL-2, culturing for 14 days, and harvesting cells. According to the method, the purity and proliferation multiple of TH2 cells are remarkably improved, the purity is 70% or above, and the proliferation multiple is 300 times or above.
本发明提供了一种体外培养Th2细胞的培养方法，属于细胞培养技术领域。获取外周血PBMC，使用含自体血浆的ALyS505N-0培养基调整细胞密度，DAY0加入CD3、CD28、BCL2L2蛋白、神经肽、吗啡、多西环素、IL-13、IL-16、IL-11、IL-4、IL-2，DAY3、DA</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Th2 cell in-vitro culture method |
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