Th2 cell in-vitro culture method

The invention provides a culture method for in-vitro culture of Th2 cells, and belongs to the technical field of cell culture. The method comprises the following steps: acquiring peripheral blood PBMC (peripheral blood mononuclear cell), adjusting cell density by using an ALyS505N-0 culture medium containing autologous plasma, adding CD3, CD28, BCL2L2 protein, neuropeptide, morphine, doxycycline, IL-13, IL-16, IL-11, IL-4, IL-2, DAY3 and DAY5 into the DAY0, adding an ALyS505N-0 culture medium containing IL-13, IL-16, IL-11, IL-4 and IL-2, respectively adding DAY7, DAY9 and DAY11 into an ALyS505N-0 culture medium containing IL-11, IL-4 and IL-2, culturing for 14 days, and harvesting cells. According to the method, the purity and proliferation multiple of TH2 cells are remarkably improved, the purity is 70% or above, and the proliferation multiple is 300 times or above. 本发明提供了一种体外培养Th2细胞的培养方法，属于细胞培养技术领域。获取外周血PBMC，使用含自体血浆的ALyS505N-0培养基调整细胞密度，DAY0加入CD3、CD28、BCL2L2蛋白、神经肽、吗啡、多西环素、IL-13、IL-16、IL-11、IL-4、IL-2，DAY3、DA