Protein standard substance determination method based on pre-column derivatization-ultra-high performance liquid chromatography

Protein standard substance determination method based on pre-column derivatization-ultra-high performance liquid chromatography

The invention relates to a protein standard substance determination method based on precolumn derivatization-ultra-high performance liquid chromatography, and belongs to the technical field of biological medicine quality control. Comprising the following steps: hydrolyzing a to-be-detected protein s...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: FAN XIAOLIN, CUI HONGEN, ZHU MAOLIN, LI SHUANG, FANG SHUAI, FENG SU, WEI HONG, LIAO DANHUA, LIU YANG, YE RENYUAN
Format: Patent
Sprache:chi ; eng
Schlagworte:
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator FAN XIAOLIN
CUI HONGEN
ZHU MAOLIN
LI SHUANG
FANG SHUAI
FENG SU
WEI HONG
LIAO DANHUA
LIU YANG
YE RENYUAN
description The invention relates to a protein standard substance determination method based on precolumn derivatization-ultra-high performance liquid chromatography, and belongs to the technical field of biological medicine quality control. Comprising the following steps: hydrolyzing a to-be-detected protein sample to form a to-be-detected free amino acid aqueous solution; preparing an internal standard working solution and a mixed standard working solution; adding the internal standard working solution and the mixed standard working solution into the obtained free amino acid aqueous solution to be detected, carrying out derivatization, and converting the free amino acid into a stable derivative; selecting a mobile phase and a chromatographic column; sequentially carrying out separation detection on the prepared mixed standard working solution and the derivative by adopting ultra-high performance liquid chromatography, and recording the signal intensity of the ultra-high performance liquid chromatography; and calculatin
format Patent
fullrecord <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN115112811A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN115112811A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN115112811A3</originalsourceid><addsrcrecordid>eNqNzL0KwjAUBeAuDqK-Q3yADFEEVymKkzi4l9vmtgnkz5sbQRdf3Vp8AKdzDnycefW-UmS0QWSGoIG0yKX99g6FRkbyNgDbGIRHNlGLFjJqMe5EKLvoig8jJPsY1WuSsjgmkMYORiSkPpKf7py9F6tFZyh64DgQJPNcVrMeXMbVLxfV-nS81WeJKTaYE3QYkJv6otROqc1eqcP2H_MB325KjQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Protein standard substance determination method based on pre-column derivatization-ultra-high performance liquid chromatography</title><source>esp@cenet</source><creator>FAN XIAOLIN ; CUI HONGEN ; ZHU MAOLIN ; LI SHUANG ; FANG SHUAI ; FENG SU ; WEI HONG ; LIAO DANHUA ; LIU YANG ; YE RENYUAN</creator><creatorcontrib>FAN XIAOLIN ; CUI HONGEN ; ZHU MAOLIN ; LI SHUANG ; FANG SHUAI ; FENG SU ; WEI HONG ; LIAO DANHUA ; LIU YANG ; YE RENYUAN</creatorcontrib><description>The invention relates to a protein standard substance determination method based on precolumn derivatization-ultra-high performance liquid chromatography, and belongs to the technical field of biological medicine quality control. Comprising the following steps: hydrolyzing a to-be-detected protein sample to form a to-be-detected free amino acid aqueous solution; preparing an internal standard working solution and a mixed standard working solution; adding the internal standard working solution and the mixed standard working solution into the obtained free amino acid aqueous solution to be detected, carrying out derivatization, and converting the free amino acid into a stable derivative; selecting a mobile phase and a chromatographic column; sequentially carrying out separation detection on the prepared mixed standard working solution and the derivative by adopting ultra-high performance liquid chromatography, and recording the signal intensity of the ultra-high performance liquid chromatography; and calculatin</description><language>chi ; eng</language><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; PHYSICS ; TESTING</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20220927&amp;DB=EPODOC&amp;CC=CN&amp;NR=115112811A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20220927&amp;DB=EPODOC&amp;CC=CN&amp;NR=115112811A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>FAN XIAOLIN</creatorcontrib><creatorcontrib>CUI HONGEN</creatorcontrib><creatorcontrib>ZHU MAOLIN</creatorcontrib><creatorcontrib>LI SHUANG</creatorcontrib><creatorcontrib>FANG SHUAI</creatorcontrib><creatorcontrib>FENG SU</creatorcontrib><creatorcontrib>WEI HONG</creatorcontrib><creatorcontrib>LIAO DANHUA</creatorcontrib><creatorcontrib>LIU YANG</creatorcontrib><creatorcontrib>YE RENYUAN</creatorcontrib><title>Protein standard substance determination method based on pre-column derivatization-ultra-high performance liquid chromatography</title><description>The invention relates to a protein standard substance determination method based on precolumn derivatization-ultra-high performance liquid chromatography, and belongs to the technical field of biological medicine quality control. Comprising the following steps: hydrolyzing a to-be-detected protein sample to form a to-be-detected free amino acid aqueous solution; preparing an internal standard working solution and a mixed standard working solution; adding the internal standard working solution and the mixed standard working solution into the obtained free amino acid aqueous solution to be detected, carrying out derivatization, and converting the free amino acid into a stable derivative; selecting a mobile phase and a chromatographic column; sequentially carrying out separation detection on the prepared mixed standard working solution and the derivative by adopting ultra-high performance liquid chromatography, and recording the signal intensity of the ultra-high performance liquid chromatography; and calculatin</description><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>PHYSICS</subject><subject>TESTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNzL0KwjAUBeAuDqK-Q3yADFEEVymKkzi4l9vmtgnkz5sbQRdf3Vp8AKdzDnycefW-UmS0QWSGoIG0yKX99g6FRkbyNgDbGIRHNlGLFjJqMe5EKLvoig8jJPsY1WuSsjgmkMYORiSkPpKf7py9F6tFZyh64DgQJPNcVrMeXMbVLxfV-nS81WeJKTaYE3QYkJv6otROqc1eqcP2H_MB325KjQ</recordid><startdate>20220927</startdate><enddate>20220927</enddate><creator>FAN XIAOLIN</creator><creator>CUI HONGEN</creator><creator>ZHU MAOLIN</creator><creator>LI SHUANG</creator><creator>FANG SHUAI</creator><creator>FENG SU</creator><creator>WEI HONG</creator><creator>LIAO DANHUA</creator><creator>LIU YANG</creator><creator>YE RENYUAN</creator><scope>EVB</scope></search><sort><creationdate>20220927</creationdate><title>Protein standard substance determination method based on pre-column derivatization-ultra-high performance liquid chromatography</title><author>FAN XIAOLIN ; CUI HONGEN ; ZHU MAOLIN ; LI SHUANG ; FANG SHUAI ; FENG SU ; WEI HONG ; LIAO DANHUA ; LIU YANG ; YE RENYUAN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN115112811A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2022</creationdate><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>PHYSICS</topic><topic>TESTING</topic><toplevel>online_resources</toplevel><creatorcontrib>FAN XIAOLIN</creatorcontrib><creatorcontrib>CUI HONGEN</creatorcontrib><creatorcontrib>ZHU MAOLIN</creatorcontrib><creatorcontrib>LI SHUANG</creatorcontrib><creatorcontrib>FANG SHUAI</creatorcontrib><creatorcontrib>FENG SU</creatorcontrib><creatorcontrib>WEI HONG</creatorcontrib><creatorcontrib>LIAO DANHUA</creatorcontrib><creatorcontrib>LIU YANG</creatorcontrib><creatorcontrib>YE RENYUAN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>FAN XIAOLIN</au><au>CUI HONGEN</au><au>ZHU MAOLIN</au><au>LI SHUANG</au><au>FANG SHUAI</au><au>FENG SU</au><au>WEI HONG</au><au>LIAO DANHUA</au><au>LIU YANG</au><au>YE RENYUAN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Protein standard substance determination method based on pre-column derivatization-ultra-high performance liquid chromatography</title><date>2022-09-27</date><risdate>2022</risdate><abstract>The invention relates to a protein standard substance determination method based on precolumn derivatization-ultra-high performance liquid chromatography, and belongs to the technical field of biological medicine quality control. Comprising the following steps: hydrolyzing a to-be-detected protein sample to form a to-be-detected free amino acid aqueous solution; preparing an internal standard working solution and a mixed standard working solution; adding the internal standard working solution and the mixed standard working solution into the obtained free amino acid aqueous solution to be detected, carrying out derivatization, and converting the free amino acid into a stable derivative; selecting a mobile phase and a chromatographic column; sequentially carrying out separation detection on the prepared mixed standard working solution and the derivative by adopting ultra-high performance liquid chromatography, and recording the signal intensity of the ultra-high performance liquid chromatography; and calculatin</abstract><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier
ispartof
issn
language chi ; eng
recordid cdi_epo_espacenet_CN115112811A
source esp@cenet
subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title Protein standard substance determination method based on pre-column derivatization-ultra-high performance liquid chromatography
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T04%3A54%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=FAN%20XIAOLIN&rft.date=2022-09-27&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN115112811A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true