Protein standard substance determination method based on pre-column derivatization-ultra-high performance liquid chromatography

The invention relates to a protein standard substance determination method based on precolumn derivatization-ultra-high performance liquid chromatography, and belongs to the technical field of biological medicine quality control. Comprising the following steps: hydrolyzing a to-be-detected protein sample to form a to-be-detected free amino acid aqueous solution; preparing an internal standard working solution and a mixed standard working solution; adding the internal standard working solution and the mixed standard working solution into the obtained free amino acid aqueous solution to be detected, carrying out derivatization, and converting the free amino acid into a stable derivative; selecting a mobile phase and a chromatographic column; sequentially carrying out separation detection on the prepared mixed standard working solution and the derivative by adopting ultra-high performance liquid chromatography, and recording the signal intensity of the ultra-high performance liquid chromatography; and calculatin