Molecular marker related to stage weight of sheep as well as detection method and application of molecular marker
The invention provides a molecular marker related to stage weight of sheep as well as a detection method and application of the molecular marker. According to the present invention, PCR amplification and sequence analysis are performed on the sheep GHR gene to discover that an A/G polymorphic site e...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention provides a molecular marker related to stage weight of sheep as well as a detection method and application of the molecular marker. According to the present invention, PCR amplification and sequence analysis are performed on the sheep GHR gene to discover that an A/G polymorphic site exists in the 196th site of the sequence represented by SEQ ID NO.1 in the amplified fragment, the KASPar primer is further used to detect the polymorphic sites of 375 Hu sheep, 31 Tan sheep and 82 Dorper sheep, the least square model is established, the correlation analysis is performed on the genotype and the stage body weight character, and the genotype and the stage body weight character are determined. Finally, it is determined that the amplified GHR gene fragment can be used as the molecular marker related to the stage weight of sheep. By detecting the molecular marker, homozygous Hu sheep with the gene being AA can be selected to enter a core group to serve as breeding sheep, and the stage weight of the sheep |
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