Multivalent activatable aptamer probe for early intelligent diagnosis of lung cancer as well as preparation and application of multivalent activatable aptamer probe

The invention belongs to the technical field of biological medicine diagnosis and detection, and particularly discloses a multivalent activatable aptamer probe for early intelligent diagnosis of lung cancer as well as preparation and application of the multivalent activatable aptamer probe. The fluorescent probe is characterized in that the 5'end of an S1 aptamer nucleic acid sequence is provided with a polyA tail and is marked with a fluorophore, and the S1 aptamer nucleic acid sequence is complementary with a complementary chain S1-CP part of which the 3 'end is marked with a fluorescence quenching group to form a double-chain structure; the multivalent activatable aptamer probe can be constructed by mixing and reacting the long DNA connecting bracket with S1-polyA and S1-CP. The preparation method of the multivalent activatable aptamer probe for early intelligent diagnosis of lung cancer is simple, convenient, time-saving and efficient; higher binding affinity and high specificity are achieved on a target;