Compositions and methods for spatial analysis of biological materials using time-resolved luminescence measurements

In alternative embodiments, compositions, including products of manufacture and kits, and methods are provided for in situ spatial analysis of biological materials such as DNA, RNA and proteins in cells, tissues and organisms, for research biology and for biomarker/drug discovery and development, and for clinical pathology and diagnosis. In alternative embodiments, compositions, including products of manufacture and kits, and methods are provided for spatially determining, visualizing, or quantifying a target biological material, including in situ staining a sample with one or more probes labeled with luminescent moieties that exhibit or encode different luminescent lifetimes (and optionally, e.g., at least one of the luminescent moieties, e.g., at least one of the luminescent moieties, e.g., at least one of the luminescent moieties, e.g., at least one of the luminescent moieties, etc. Spectral) features; and then time-resolved luminescence imaging, measurement and analysis are carried out. 在替换实施方式中，提供了组合物，包括