Methods for cell-free protein expression

A method for cell-free expression of peptides or proteins in a liquid-filled digital microfluidic device is disclosed. Droplets with components required for cell-free protein expression may be manipulated by electrical motion in order to increase the level of protein expression in the droplet. 公开了一种用于在液体填充的数字微流体装置中肽或蛋白质的无细胞表达的方法。具有无细胞蛋白质表达所需组分的液滴可以通过电运动来操纵，以便提高液滴中蛋白质表达的水平。