Homogeneous immunodetection kit for Alzheimer's disease

The invention discloses a homogeneous immunodetection kit for Alzheimer's disease, and belongs to the technical field of biological detection. The influence of high-abundance protein and non-specific interfering substances is reduced through dilution, meanwhile, the sample size 50-100 times lar...

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Bibliographische Detailangaben
Hauptverfasser: MO HONGFENG, CHEN HAILEI, OUYANG DONGYUN, LONG XIANHUA, LIANG BINGSHAO, YANG WEICHAO
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a homogeneous immunodetection kit for Alzheimer's disease, and belongs to the technical field of biological detection. The influence of high-abundance protein and non-specific interfering substances is reduced through dilution, meanwhile, the sample size 50-100 times larger than that of other instruments is adopted, and the sensitivity is improved by enriching antigens in blood. By means of the enrichment method, a high-speed full-automatic biochemical analyzer can be used for detection, and expensive special instruments do not need to be purchased for detection of the project. A homogeneous immune washing-free reaction is adopted, after a semi-enzyme labeled double antibody and a to-be-detected antigen are combined, due to the fact that space of semi-enzyme is close to each other, the semi-enzyme can be complementarily combined to form an active enzyme, then a multi-enzyme coupling amplification effect is utilized, the effect of a catalytic substrate is enhanced, and a color developme