Detection and analysis method of cytokine fusion protein

The invention relates to the technical field of biological analysis, in particular to a detection and analysis method of cytokine fusion protein, which comprises the following steps: S1, preparing an immobilized antigen; s2, diluting the sample to be detected, and incubating for 40-80 minutes; s3, carrying out antigen affinity capture on the sample to be detected to form an antibody-cytokine fusion protein immune complex, and washing the plate after the antigen affinity capture is finished; and S4, specifically binding a ruthenium-labeled anti-cytokine fusion protein antibody with the cytokine fusion protein part of the immune complex to form an antibody-cytokine fusion protein-labeled antibody complex. Incubating for 40 to 80 minutes at the room temperature at 500 rpm; s5, an MSDReadBuffer is added after the board is washed, and a signal is read on the MSDQuickPlexSQ120, and the MSDReadBuffer is added on the MSDQuickPlexSQ120; and S6, analyzing software data to obtain pharmacokinetic parameters of the cytoki