Genetically engineered bacterium capable of degrading feathers as well as construction method and application of genetically engineered bacterium
The invention discloses a genetic engineering bacterium capable of degrading feathers as well as a construction method and application of the genetic engineering bacterium, and relates to the technical field of genetic engineering, a keratinase gene Y115990 in a feather degrading bacterium Bacillus...
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| creator | LI KUNTAI TAN MINGHUI ZHANG JUN ZHANG RONG PENG SHUAIYING |
| description | The invention discloses a genetic engineering bacterium capable of degrading feathers as well as a construction method and application of the genetic engineering bacterium, and relates to the technical field of genetic engineering, a keratinase gene Y115990 in a feather degrading bacterium Bacillus sp.JY-23 (the preservation number is CCTCC NO: M 20211638) screened in the earlier stage is cloned and heterologous expressed, a keratinase recombinant expression engineering bacterium Escherichia. ColiBL21 (DE3)/pET-22b (+)-Y115990 is successfully constructed, and the genetic engineering bacterium can be used for degrading feathers. The bacterial strain can directly degrade feathers under the condition that the feathers are used as the unique carbon and nitrogen source, and efficiently express keratinase, so that compared with the traditional engineering bacteria which take carbohydrate carbon sources and organic nitrogen sources as culture medium components for fermentation, the engineering bacteria have the adva |
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ColiBL21 (DE3)/pET-22b (+)-Y115990 is successfully constructed, and the genetic engineering bacterium can be used for degrading feathers. 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ColiBL21 (DE3)/pET-22b (+)-Y115990 is successfully constructed, and the genetic engineering bacterium can be used for degrading feathers. 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ColiBL21 (DE3)/pET-22b (+)-Y115990 is successfully constructed, and the genetic engineering bacterium can be used for degrading feathers. The bacterial strain can directly degrade feathers under the condition that the feathers are used as the unique carbon and nitrogen source, and efficiently express keratinase, so that compared with the traditional engineering bacteria which take carbohydrate carbon sources and organic nitrogen sources as culture medium components for fermentation, the engineering bacteria have the adva</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FODDER FOODS OR FOODSTUFFS HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE |
| title | Genetically engineered bacterium capable of degrading feathers as well as construction method and application of genetically engineered bacterium |
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