Genetically engineered bacterium capable of degrading feathers as well as construction method and application of genetically engineered bacterium

The invention discloses a genetic engineering bacterium capable of degrading feathers as well as a construction method and application of the genetic engineering bacterium, and relates to the technical field of genetic engineering, a keratinase gene Y115990 in a feather degrading bacterium Bacillus...

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Bibliographische Detailangaben
Hauptverfasser: LI KUNTAI, TAN MINGHUI, ZHANG JUN, ZHANG RONG, PENG SHUAIYING
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a genetic engineering bacterium capable of degrading feathers as well as a construction method and application of the genetic engineering bacterium, and relates to the technical field of genetic engineering, a keratinase gene Y115990 in a feather degrading bacterium Bacillus sp.JY-23 (the preservation number is CCTCC NO: M 20211638) screened in the earlier stage is cloned and heterologous expressed, a keratinase recombinant expression engineering bacterium Escherichia. ColiBL21 (DE3)/pET-22b (+)-Y115990 is successfully constructed, and the genetic engineering bacterium can be used for degrading feathers. The bacterial strain can directly degrade feathers under the condition that the feathers are used as the unique carbon and nitrogen source, and efficiently express keratinase, so that compared with the traditional engineering bacteria which take carbohydrate carbon sources and organic nitrogen sources as culture medium components for fermentation, the engineering bacteria have the adva