High-membrane-column-flux cell perfusion culture membrane column system and method and application thereof

The invention discloses a high-membrane-column-flux cell perfusion culture membrane column system which is characterized in that a high-flux membrane column comprises a buffer column and a main membrane column which are communicated with each other, a pulse cavity communicated with the main membrane column is arranged at the lower part of the main membrane column, a high-frequency pulser is arranged on the pulse cavity, and the high-frequency pulser is used for performing periodic high-frequency pulse compression or expansion on the pulse cavity. When fluid in the system flows forwards (from the lower end to the upper end of the membrane column), a filtration medium is mainly filtered out from the outer cavity of the lower-end main membrane column, when the fluid flows backwards (from the upper end to the lower end of the membrane column), the filtration medium is mainly filtered out from the outer cavity of the upper-end buffer column, and part of filtrate enters the lower-end main membrane column through th