Method for reducing allergenicity of tropomyosin

The invention discloses a method for reducing allergenicity of tropomyosin, and belongs to the technical field of biology. The method for reducing the allergenicity of the tropomyosin comprises the following steps: diluting the tropomyosin with water until the concentration is 2mg/mL, adding pyruvic aldehyde, heating in a water bath for 15 minutes, modifying, and then rapidly cooling a reaction system to 4 DEG C or below so as to reduce the allergenicity of the tropomyosin. According to the method, amino acids such as lysine, arginine, aspartic acid and glutamine in the tropomyosin can be modified by pyruvic aldehyde, the tropomyosin is promoted to be crosslinked under the heating condition, protein molecule aggregates are generated, alpha-helix and beta-folding structures are reduced, and finally the sensitization of the tropomyosin is reduced. 本发明公开一种降低原肌球蛋白致敏性的方法，属于生物技术领域。所述降低原肌球蛋白致敏性的方法为：将原肌球蛋白加水稀释至浓度为2mg/mL，加入丙酮醛，水浴加热15min，进行修饰，然后将反应体系迅速降温至4℃以下，以降低原肌球蛋白的致敏性。上述方法可使原肌球蛋白中的赖氨酸、精氨酸、天冬氨酸和谷氨酰胺等氨基酸被丙酮醛修饰，并在加热条件