Multiplex immunofluorescence detection of target antigens

A multispectral immunofluorescence imaging method of a biological sample is described. The methods allow for the simultaneous direct detection of seven or more target antigens using directly labeled antibody fluorophore conjugates. The method enables multiplex detection and analysis of multiple biomarkers simultaneously across a planar biological sample, thereby providing unique spatio-temporal insight in immunotherapy and immunodiagnosis. 描述了一种生物样本的多光谱免疫荧光成像方法。所述方法允许使用直接标记的抗体荧光团缀合物同时直接检测七种或更多种靶抗原。所述方法使得能够在整个平面生物样本上同时多重检测和分析多种生物标志物，从而在免疫治疗和免疫诊断中提供独特的时空洞察力。