Application of guar gum, fluorescence enhanced gold nanocluster and alpha-glucosidase detection and enzyme inhibitor screening method

The invention provides an application of guar gum, a fluorescence enhanced gold nanocluster and alpha-glucosidase detection method and an enzyme inhibitor screening method, and belongs to the technical field of nano biosensing. The invention provides an application of guar gum in improving the fluorescence intensity of gold nanoclusters. In the invention, the guar gum is a natural high-molecular polymer extracted from guar seeds of a leguminous plant, and has the advantages of low price, no toxicity and good biocompatibility; guar gum is mainly composed of galactose and mannose, a molecular skeleton of the guar gum is rich in a large number of hydroxyl groups, the fluorescence emission intensity of the gold nanocluster can be improved, on the basis, alpha-glucosidase activity detection and alpha-glucoside inhibitor screening can be achieved with high sensitivity and low cost, and the guar gum is environmentally friendly, easy to operate and suitable for industrial production. Modification of a fluorescent pro