Minimally invasive blood extraction method and chromosome slide preparation method for trionychidae animals

The invention discloses a minimally invasive blood extraction method and a chromosome slide preparation method for trionychidae animals, and belongs to the technical field of cytogenetics, the method comprises the following steps: S01, placing a to-be-detected animal in an aquarium system at 30 DEG C for temporary rearing for more than or equal to 10 days; s02, performing PHA injection on the animal to be detected by adopting an intraperitoneal injection method. And S03, extracting blood of the sample to be detected. S04, mixing the anticoagulant solution with the colchicine solution to obtain a mixture A; s05, mixing the mixture A with a lymphocyte separating agent to obtain a mixture B; and S06, in the mixture B, sucking the lymphocyte supernatant, adding normal saline, uniformly mixing, and centrifuging to obtain lymphocyte precipitate. And S07, re-suspending lymphocyte precipitates by using a 0.5% KCl hypotonic solution, performing hypotonic treatment at 37 DEG C for 50 minutes, and centrifugally collecti